Osaka University Graduate School of Medicine

3 ARTICLES PUBLISHED IN JoVE

Medicine

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Yujiro Naito¹, Akio Osa¹, Kentaro Masuhiro¹, Takashi Hirai¹, Shohei Koyama¹, Atsushi Kumanogoh¹

¹Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine

We developed a simple flow cytometry assay for evaluating the binding of PD-1–blocking antibodies to T cells, requiring only a drop of peripheral blood from cancer patients.

Neuroscience

Reliable Acquisition of Electroencephalography Data during Simultaneous Electroencephalography and Functional MRI

Hui Ming Khoo¹, Yuya Fujita¹, Naoki Tani¹, Tetsuya Shimokawa², Natalja Zazubovits³, Satoru Oshino¹, Jean Gotman³, Haruhiko Kishima¹

¹Department of Neurosurgery, Osaka University Graduate School of Medicine, ²Center for Information and Neural Networks, National Institute of Information and Communications Technology, ³Montreal Neurological Institute and Hospital, McGill University

This article provides a straightforward protocol for acquiring good quality electroencephalography (EEG) data during simultaneous EEG and functional magnetic resonance imaging by utilizing readily available medical products.

Biology

Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes

So Goto^1,2,3, Michael Frost⁴, Christine Wildsoet¹

¹Herbert Wertheim School Optometry and Vision Science, University of California, Berkeley, ²Department of Ophthalmology, Osaka University Graduate School of Medicine, ³Department of Ophthalmology, Tokyo Medical Center, National Hospital Organization, ⁴Department of Optometry and Vision Science, School of Optometry, University of Alabama at Birmingham

We describe a simple and efficient method for isolating cells of the retinal pigment epithelium (RPE) cells from the eyes of young pigmented guinea pigs. This procedure allows for follow-up molecular biology studies on the isolated RPE, including gene expression analyses.